Sensitivity of Detecting Alpha-Synuclein Accumulation in the Gastrointestinal Tract and Tissue Volume Examined

Article information

J Mov Disord. 2022;15(3):264-268

Publication date (electronic) : 2022 July 26

doi : https://doi.org/10.14802/jmd.22042

Chaewon Shin¹, Seong-Ik Kim², Sung-Hye Park², Jung Hwan Shin³, Chan Young Lee⁴, Han-Kwang Yang⁵, Hyuk-Joon Lee⁵, Seong-Ho Kong⁵, Yun-Suhk Suh⁶, Han-Joon Kim³, Beomseok Jeon^,3

¹Department of Neurology, Chungnam National University Sejong Hospital, Chungnam National University College of Medicine, Sejong, Korea

²Department of Pathology, Seoul National University College of Medicine, Seoul, Korea

³Department of Neurology, MRC and Movement Disorder Center, Seoul National University Hospital, Parkinson Study Group, Seoul National University College of Medicine, Seoul, Korea

⁴Department of Neurology, Ehwa Womans University Mokdong Hospital, Seoul, Korea

⁵Department of Surgery, Cancer Research Institute, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Korea

⁶Department of Surgery, Seoul National University Bundang Hospital, Seongnam, Korea

Corresponding author: Beomseok Jeon, MD, PhD Department of Neurology, MRC and Movement Disorder Center, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul 03080, Korea / Tel: +82-2-2072-2876 / Fax: +82-2-3672-7553 / E-mail: brain@snu.ac.kr

Received 2022 March 15; Revised 2022 May 23; Accepted 2022 May 31.

Abstract

MATERIALS & METHODS

RESULTS

Nine patients (4 PD and 5 iRBD) and five controls were selected. In previous studies, pAS was positive in 5/9 patients (55.6%) and 1/5 controls (20.0%). In an extensive evaluation of 10 slides per patient, the positivity rate increased to 8/9 patients (88.9%), but the rate remained the same (20.0%) in controls (p = 0.023; Table 1 and Figure 1).

Table 1.

pAS immunostaining results of extensive tissue (5 slides per block) compared with original staining findings

Figure 1.

pAS immunostaining results of extensive tissue evaluation (five sequential slides per block) compared with original staining findings. A: The first and second columns compare the pAS positivity results from previous studies (I) [6,8] with the current extensive evaluation (E). The pAS staining results of distal and proximal blocks are also presented. When discordant results were obtained between proximal and distal blocks, only mild positive findings (semiquantitative grade 1) were present (i.e., the three subjects P06, P08, and C02). Moreover, pAS positivity was not found in all five sequential slides in two subjects (P02 and C02), among whom the semiquantitative grade was also 1+. B: Representative figures of multifocally distributed and differently rated nerve plexuses in one patient (P07). Red arrows indicate pAS-positive findings. Calibration bars: figures subtitled Negative, Grade 2+, and Grade 3+ = 50 μm; figure subtitled Grade 1+ = 80 μm. pAS, phosphorylated alpha-synuclein.

In the detailed analysis of 5 slides from the proximal and distal blocks in both the patient and control groups, samples from 6 of 9 subjects with pAS positivity (66.7%) were positive in both proximal and distal blocks (Supplementary Table 1 in the online-only Data Supplement and Figure 1). Moreover, positivity was present in all 5 serial sections in 80.0% of positively stained blocks (12/15); three blocks of P02 and C02 were exceptions.

Regarding the severity of pAS positivity, severe density (3+) was found in both PD and iRBD patients. When the results were discordant between proximal and distal blocks (P06, P08, and C02), only mild density (1+) was found in the positive block. Similarly, when pAS-positive findings were not present in all 5 serial sections (P02 and C02), only mild density (1+) was seen. Finally, there was a severity gradient in the distal block of P01.

DISCUSSION

This study demonstrates that pAS positivity increases with increasing tissue volume examined, which is consistent with previous studies. Beach et al. [2] reported that the pAS positivity rate in the GI tract increased from 11/17 (64.7%) to 14/15 (93.3%) when multiple slides and 80-μm frozen sections were examined in their large-scale autopsy study. Lebouvier et al. [3-5] also reported good results (72%–80%, pAS positivity) using wholemount staining, which involves microdissecting the submucosa from the mucosa in the biopsied colon tissue and mounting and staining the whole submucosa at once. The method allowed us to evaluate a large submucosal tissue volume. This study also showed that mild AS accumulation with the semiquantitative grade 1+ could be inconsistently rated within the proximal and distal sites of one organ and consecutive sections taken from a single site. These findings could be explained by the multifocal distribution of AS accumulation in the GI tract, which has a greater influence on the pathologic evaluation of milder disease stages. Therefore, an extensive volume of large full-depth pathologic specimens is required to achieve the maximal positivity rate, but this is not feasible in clinical practice. These results further support the fundamental limit of biopsies that contain only a small portion of mucosal and submucosal layers from the intestinal wall [1].

There were no definite differences in the distribution and severity between patients with iRBD and PD, although iRBD is a prodromal stage of PD. Previous studies have reported pAS-positive rates of 62.5% and 58.3% in the stomach specimens of patients with iRBD and PD, respectively [1,6]. Several studies have reported that the sensitivity of detecting AS accumulation is higher in patients with iRBD than in PD using specimens from the colon [10], submandibular gland [11], and skin [12]. These results support the notion that AS accumulation in the enteric nervous system precedes the progression of Lewy pathology from the periphery. Therefore, it supports the gut-to-brain progression model presented in Braak’s hypothesis [13].

In contrast, one patient with iRBD (P09) showed no positivity on extensive examination. There should be pathologic changes in the brain because iRBD was confirmed with polysomnography. This result suggests an alternative progression pathway for synucleinopathy that does not follow the gut-to-brain progression model. One study examined AS accumulation in the stomach and vagus nerve from an autopsied series of normal controls, patients with incidental Lewy body disease and patients with PD [14]. There was no AS accumulation in the stomach or vagus nerve in normal elderly subjects in that study, which indicated that AS accumulation in the enteric nervous system was present only in patients who had Lewy pathology in their brain. Therefore, the result questioned the so-called “body-first” origin of synucleinopathy. Recently, a “brain-first” PD hypothesis, in which pathologic AS descends from the brain to the gut, has been suggested based on multimodal imaging data [15,16]. Taken together, this study provides evidence for complexity in the origin and progression of Lewy pathology in synucleinopathy. Another possibility is that some patients with iRBD do not progress to neurodegenerative diseases for a long period of time [17] or develop non-Lewy body diseases, such as multiple system atrophy [18]. Therefore, this patient might have had non-Lewy pathology in the brain.

The negative result of one control subject (C01) whose initial result was positive can be explained by the different definitions of pAS positivity in the previous study [1]. The previous definition of pAS positivity allowed a ‘diffuse staining’ pattern that the initial staining result for this subject showed. Later, we used a more conservative definition [6] based on evidence from subsequent studies [7,8]. The staining results for this subject confirmed that the current definition of pAS positivity used in this study was more reliable than the previous one because there were no positive findings in this subject, including the ‘diffuse staining’ pattern.

In conclusion, examination of a large tissue volume increased the sensitivity of detecting AS accumulation in the GI tract. This study implies a fundamental limit of biopsied tissue and highlights the complexity of pathologic progression of synucleinopathy.

Supplementary Materials

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